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Serum (A) total T3 and (B) <t>total</t> <t>T4</t> levels in 5 mo and 13 mo male WT control, ZSF1 lean control, and ZSF1 obese rats; Serum (C) total T3 and (D) total T4 levels in 5 mo, 13 mo and 20 mo male WT control and ZSF1 lean control. All values are analyzed using Two-way ANOVA (TWA) and presented as means ± standard deviation; WT: Wild Type; T4: Thyroxine; T3: Triiodothyronine; *p<0.05; ****p<0.0001 vs 5 mo WT control (TWA); #### p < 0.0001 vs 5 mo ZSF1 lean control (TWA); ΦΦ p<0.01vs 13 mo ZSF1 lean control (TWA); θ p<0.05 vs. 13 mo obese HFpEF (t-test).
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Serum (A) total T3 and (B) <t>total</t> <t>T4</t> levels in 5 mo and 13 mo male WT control, ZSF1 lean control, and ZSF1 obese rats; Serum (C) total T3 and (D) total T4 levels in 5 mo, 13 mo and 20 mo male WT control and ZSF1 lean control. All values are analyzed using Two-way ANOVA (TWA) and presented as means ± standard deviation; WT: Wild Type; T4: Thyroxine; T3: Triiodothyronine; *p<0.05; ****p<0.0001 vs 5 mo WT control (TWA); #### p < 0.0001 vs 5 mo ZSF1 lean control (TWA); ΦΦ p<0.01vs 13 mo ZSF1 lean control (TWA); θ p<0.05 vs. 13 mo obese HFpEF (t-test).
Spinal Stimulator With Two Eight Contact Electrodes At Levels T6 And T7 And T3 T4, supplied by Boston Scientific Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Serum (A) total T3 and (B) <t>total</t> <t>T4</t> levels in 5 mo and 13 mo male WT control, ZSF1 lean control, and ZSF1 obese rats; Serum (C) total T3 and (D) total T4 levels in 5 mo, 13 mo and 20 mo male WT control and ZSF1 lean control. All values are analyzed using Two-way ANOVA (TWA) and presented as means ± standard deviation; WT: Wild Type; T4: Thyroxine; T3: Triiodothyronine; *p<0.05; ****p<0.0001 vs 5 mo WT control (TWA); #### p < 0.0001 vs 5 mo ZSF1 lean control (TWA); ΦΦ p<0.01vs 13 mo ZSF1 lean control (TWA); θ p<0.05 vs. 13 mo obese HFpEF (t-test).
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Serum (A) total T3 and (B) <t>total</t> <t>T4</t> levels in 5 mo and 13 mo male WT control, ZSF1 lean control, and ZSF1 obese rats; Serum (C) total T3 and (D) total T4 levels in 5 mo, 13 mo and 20 mo male WT control and ZSF1 lean control. All values are analyzed using Two-way ANOVA (TWA) and presented as means ± standard deviation; WT: Wild Type; T4: Thyroxine; T3: Triiodothyronine; *p<0.05; ****p<0.0001 vs 5 mo WT control (TWA); #### p < 0.0001 vs 5 mo ZSF1 lean control (TWA); ΦΦ p<0.01vs 13 mo ZSF1 lean control (TWA); θ p<0.05 vs. 13 mo obese HFpEF (t-test).
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TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine <t>(T4),</t> 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating <t>hormone</t> <t>(TSH).</t> (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.
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TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine <t>(T4),</t> 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating <t>hormone</t> <t>(TSH).</t> (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.
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TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine <t>(T4),</t> 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating <t>hormone</t> <t>(TSH).</t> (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.
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Arbor Assays t4 levels
TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine <t>(T4),</t> 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating <t>hormone</t> <t>(TSH).</t> (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.
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TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine <t>(T4),</t> 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating <t>hormone</t> <t>(TSH).</t> (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.
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Serum (A) total T3 and (B) total T4 levels in 5 mo and 13 mo male WT control, ZSF1 lean control, and ZSF1 obese rats; Serum (C) total T3 and (D) total T4 levels in 5 mo, 13 mo and 20 mo male WT control and ZSF1 lean control. All values are analyzed using Two-way ANOVA (TWA) and presented as means ± standard deviation; WT: Wild Type; T4: Thyroxine; T3: Triiodothyronine; *p<0.05; ****p<0.0001 vs 5 mo WT control (TWA); #### p < 0.0001 vs 5 mo ZSF1 lean control (TWA); ΦΦ p<0.01vs 13 mo ZSF1 lean control (TWA); θ p<0.05 vs. 13 mo obese HFpEF (t-test).

Journal: bioRxiv

Article Title: Noncoding and Coding Mechanisms of Aging Heart Failure with Preserved Ejection Fraction with Thyroid Dysfunction

doi: 10.1101/2025.02.04.636476

Figure Lengend Snippet: Serum (A) total T3 and (B) total T4 levels in 5 mo and 13 mo male WT control, ZSF1 lean control, and ZSF1 obese rats; Serum (C) total T3 and (D) total T4 levels in 5 mo, 13 mo and 20 mo male WT control and ZSF1 lean control. All values are analyzed using Two-way ANOVA (TWA) and presented as means ± standard deviation; WT: Wild Type; T4: Thyroxine; T3: Triiodothyronine; *p<0.05; ****p<0.0001 vs 5 mo WT control (TWA); #### p < 0.0001 vs 5 mo ZSF1 lean control (TWA); ΦΦ p<0.01vs 13 mo ZSF1 lean control (TWA); θ p<0.05 vs. 13 mo obese HFpEF (t-test).

Article Snippet: Serum total T3 (tT3) and total T4 (tT4) levels were analyzed using respective Accubind enzyme-linked immuno-sorbent assay (ELISA) kits (Monobind Inc., USA) with appropriate controls, following manufacturer instructions.

Techniques: Control, Standard Deviation

TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine (T4), 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating hormone (TSH). (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.

Journal: iScience

Article Title: TRIAC disrupts cerebral thyroid hormone action via negative feedback and heterogenous distribution among organs

doi: 10.1016/j.isci.2023.107135

Figure Lengend Snippet: TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine (T4), 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating hormone (TSH). (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.

Article Snippet: We measured serum free T4 levels using an FT3, FT4, TSH AccuLite VAST CLIA kit (Monobind Inc., Lake Forest, CA) with Spark (Tecan, Zurich, Switzerland).

Techniques: Staining, Quantitation Assay